Studying the Effect of Cell Therapy by Endothelial Progenitor Cells With and Without Heme Oxygenase Inducers in Induced Pulmonary Hypertension Rat Model, MOHAMMED T. ABDELAZIZ, LAILA A. RASHED, DOAA M. GHARIB, RANIA EL-SAYED and RADWA M. GABER
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- Category: Vol. 83, March 2015
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Studying the Effect of Cell Therapy by Endothelial Progenitor Cells With and Without Heme Oxygenase Inducers in Induced Pulmonary Hypertension Rat Model, MOHAMMED T. ABDELAZIZ, LAILA A. RASHED, DOAA M. GHARIB, RANIA EL-SAYED and RADWA M. GABER
Abstract
Purpose: We investigated whether or not intravenous injection of bone marrow-derived endothelial progenitor cells (EPCs), alone or combined with Heme-oxygenase (HO) inducer can restore pulmonary hemodynamics and increase microvascular perfusion in the rat monocrotaline (MCT) model of pulmonary artery hypertension (PAH) so improve right ventricle performance.
Material and Methods: Rats were divided into control, rats with PAH, PAH rats receiving EPCs, PAH rats receiving HO-1 inducer (cobalt protoporphyrin) and PAH rats receiving EPCs plus HO-1 inducer. After 1 month, right ventricular function, was assessed. Gene expression of pro ANP, SDF-1, Bax/Bcl-2, MMP-9 were studied.
Results: Heart functions showed improvement in all the treated groups compared to PAH group. Gene expression demonstrated that proANP, MMP-9 and Bax were downregu-lated while Bcl-2 and SDF-1 were upregulated.
Conclusion: The present study proved that, administration of BM-EPCs alone, HO-1 alone or EPCs plus HO-1 produce therapeutic effect in restoring pulmonary hemodynamics and so improving right ventricle performance in PAH. This effect may be through antiapoptotic action of EPCs and HO-1.