Monocyte Chemoattractant Protein-1 (MCP-1), Chemokine Receptor2 Gene Polymorphism and Level of MCP-1 in Behcet's Disease: A Case-Control Study
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- Category: Vol. 90, December 2022
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Monocyte Chemoattractant Protein-1 (MCP-1), Chemokine Receptor2 Gene Polymorphism and Level of MCP-1 in Behcet's Disease: A Case-Control Study, AMAL H. EISSA, HEBA M. SELIM, HUSSEIN S. EL-FISHAWY, KARAM K. NAGUIB, MOHAMED S. TAWFIK and ABEER M. ZAHRAN
Abstract
Bachground: Behcet’s disease (BD) is chronic autoimmune vasculitic disease, its pathogenesis still unclear, it could be a combination of environmental and genetic factors. Both MCP-1 and its receptor CCR2 have been incriminated in the pathogenesis of multiple inflammatory disorders. Aim of Study: The aim of this study wasto investigate the potential associationof serum of monocyte chemoattractant protein-1 (MCP-1), genetic derangement of MCP-1 and chem-okine receptor2 (CCR2) with Behcet's disease. Patients and Methods: Thirty BD patients' blood samples were gathered and 30 healthy subjects with matching age and sexa, were tested for blood MCP-1 using Enzyme linked Immunosorbant assay (ELISA), MCP-1 c. 2518A/G and CCR2 -V641 polymorphism as determined by polymerase chain reaction (PCR). Assessment of disease activity was done using Behcet's Disease Current Activity Form (BDCAF). Results: The studied patients mean age was 34.9±12.2 years, mean age of disease onset was 26.7±8.8 years and mean disease duration 6.9±7.3 years. Blood levels of MCP-1 in BD patients was 241.7±179.45mg/l versus 23.1±27.06mg/l in healthy subjects and the difference between both groups was significantly high (p<0.0001). MCP-1 was positively correlated with disease activity, but it did not reach statistically significant value (p>0.05). Level of MCP-1 was higher in subjects with heterozygous MCP-1 and CCR2 genes (310±247.74mg/l) compared to subjects with normal MCP-1 and CCR2 genes and the difference was significantly high (p<0.0001). MCP-1 gene polymorphism was positive in 30% of cases versus 16.1% in healthy controls with (p>0.05). Heterozygous CCR2 gene was positive in 9 Behcet'sdisease patients and in 5 of the control group (p>0.05). Conclusion: MCP-1 serum level was significantly higher in BD cases in comparison to control group, in addition it was significantly high in subjects with heterozygous MCP-1 and CCR2 compared to those with normal one. This could providemore understanding of BD pathogenesis and suggest new therapeutic modalities.