Carbapenem Resistance among Clinical Isolates of Enterobacteriaceae,
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- Category: Vol. 84, March 2016
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Carbapenem Resistance among Clinical Isolates of Enterobacteriaceae, MAHA M. KOTB, NAGWA SEDKY, EMAN A. EL-SEIDI, ALAA R. AWAD and SAHAR S. AL-WAKEEL
Abstract
Aim: This study aimed to determine the presence of carbapenem resistance among Enterobacteriaceae by disk diffusion method and Epsilometer test (E-test).
Methodology: In the present study, 100 Enterobacteriaceae were isolated from different clinical samples and identified by Analytical Profile Index (API) 20 E. Antimicrobial suscep-tibility testing was done by modified kirby bauer disk diffusion method; using carbapenems; Ertapenem (ETP) and Meropenem (MEM) and third generation cephalosporins; Cefoprazone (CFP) and Cefotaxime (CTX) and followed by Minimum Inhibitory Concentration (MIC) determination by E-test for ETP and MEM.
Results: The disk diffusion method showed that 8 1 % of the isolates were resistant to CTX, 79% were non-susceptible (i.e. resistant and intermediate isolates) to CFP, and the carbapenem non-susceptibility was 68 isolates (68%) to ETP and 57 isolates (57%) to MEM. MIC determination results by E-test method differed from disk diffusion results and showed that the carbapenem non-susceptibility to ETP and MEM was 19% and 9% respectively.
Conclusions: The presence of carbapenem resistance among Enterobacteriaceae isolates causing healthcare-associated infections in the current study emphasizes the necessity for early detection of these isolates and reporting to infection prevention staff to overcome their spread.