Effect of Different Thawing Temperatures on the Motility Recovery of Cryopreserved Human Sperm, ASHRAF FAYEZ, SARWAT AL-AHWANI, AHMED EL-GUINDI, AMR ALAHWANI, SHADY ZAKI and ISLAM FATHY SOLIMAN ABD EL-RAHMAN
Abstract
Introduction: standard protocol designed to optimize sperm motility recovery after thawing has not yet been established.
Purpose: We aimed to study the effect of different thawing temperatures on the motility recovery of cryopreserved human sperm.
Patients and Methods: Fifty semen samples of males complaining of primary infertility and oligoasthenoteratozoospeтrniа. A semen sample was obtained and evaluated. Every sample was divided into three straws that underwent cryopreservation, then thawing at three different temperatures; 25°C, 37°C and 40°. Pre-cryopreservation sperm motility was compared to the post-thawing motility at each of the three thawing temperatures.
Results: Total motility percentage was better at thawing temperatures of 37°C and 40°C compared to 25°C. Rapidly progressive motility was the highest at thawing temperature 40°C compared to the other thawing temperatures; 25°C and 37°C as p-value was 0.08, <0.001 and <0.001 respectively denoting no significant difference in change of the rapidly progressive motility before cryopreservation and at thawing temperature 40°C.
Conclusion: Thawing cryo-preserved semen samples with severe oligoasthenoteratozoospermia at 40°C provides highest potential for restoring sperm motility.