16s and 23s RRNA ITS Molecular Identification of Acinetobacter Isolates in Assir Region, Kingdom of Saudi Arabia
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- Category: Vol. 81, September 2013
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16s and 23s RRNA ITS Molecular Identification of Acinetobacter Isolates in Assir Region, Kingdom of Saudi Arabia, NAZAR M. ABDALLA, AMANI A. OSMAN, WALEED O. HAIMOUR, MOHAMMED A.A. SARHAN, MOHAMMED N. MOHAMMED, EYHAB M. ZYAD and ABDALLA M. ALGHTANI
Abstract
Aim: This study aim at molecular identification of 16s and 23s rRNA ITS of Acinetobacter clinical isolates in Assir region.
Study Design: Cross sectional cohort study of Acineto-bacter clinical isolates in Assir region. This study is funded by the college of scientific research, King Khalid University, Abha, Saudi Arabia. Ref. KKU-MED-11-017 (The first sci-entific research funded program of KKU).
Study Period: Dec. 2011- Dec. 2012.
Methods: 150 Acinetobacter clinical isolates in Assir central hospital presented with variable clinical manifestations were included. Variable samples including; sputum, urine, blood and skin were collected. Cases were confirmed by bacteriological and biochemical tests including, gram stain, culture and API20 were used. DNA extraction from cultured samples were collected and 16s and 23s rRNA ITS specific primers were run in PCR for molecular identification.
Results: PCR products of the 16s and 23s rRNA ITS specific primers have detect 145 positive isolates from all 150 Acinetobacter clinical isolates, detection rate of 96.7%.
Conclusion: The rapid and accurate identification of clinically significant Acinetobacter strains by molecular tools will improve insight into their epidemiology and allow for targeted therapeutic and infection control measures against clinically important strains. The identification genomic rate is high 96.7% while at species level is poor.