Effect of Some Fruits and Vegetables Peels Extract on CCl4 Induced Hepatic Injury in Rats
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- Category: Vol. 79, March 2011
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Effect of Some Fruits and Vegetables Peels Extract on CCl4 Induced Hepatic Injury in Rats,MOHAMED S. ABD EL-BAKY and FATMA L. AHMED
Abstract
Free radicals generated by hepatotoxins like CCl4 may overpower the mechanism of the liver and cause hepatic damage. Though the modern medicinal system has grown phenomenally, the drug for treating hepatic disease is still a dream. Hence, people are looking at traditional systems of medicines for remedies to hepatic disorders. Phenolic com-pounds can act as antioxidants by many potential pathways such as free radical-scavenging, oxygen radical absorbance, and chelating of metal ions.
Aim: The present study was performed to evaluate anti-oxidants compounds of some fruit and vegetable peels extract (red apple, banana, red beet roots, and potatoes) and its effects on CCl4 induced hepatic injury in rats.
Methods: Extraction yield of phenolic compounds by different solvents (methanol, ethanol and diethyl ether), esti-mation of total phenolics, total flavonoids and total flavonols of red apple peels, banana peels, red beet peels and potato peels were estimated. A total of thirty six male healthy rats, weighing between (200-220gm) were divided into six groups. To induction cirrhosis all rats except control negative were subcutaneous injected by carbon tetrachloride CCl4 which diluted by paraffin oil (1:1) [in a dose of 2ml/kg of body weight of rat], twice in the week during the experimental feeding period, each group containing 6 rats. Control groups (1,2) negative and positive were fed on basal diet without supplementation. All treated cirrhotic groups (3-6) were fed on basal diet and administrated by gastric tube 2g/kg/day methanol extract of (apple peels methanol extract (APME), banana peels methanol extract (BPME), red beet peels methanol extract (RBPME) and potato peels methanol extract (PPME) respectively.
Results: The results found that Methanol extracts of tested sample proved that the best solvent for yield extracts of all tested solvents, also there was found a strong positive corre-lation between yield extracts of tested samples and polyphenols content. The results observed that cirrhotic rats (control positive group) had significant increase in serum liver function and kidney function parameters AST, ALT, uric acid, urea and creatinine) comparing with control negative group. Also, it could be observed that all cirrhotic groups administrated with different methanol extract of some fruits and vegetables peels (apple APME, banana BPME, red beet RBPME and potato PPME) had significant decrease in serum liver and kidney function parameters and protective effect against histopatho-logical hepatotoxicity induced by CCl4 comparing with control positive. The best treatment was apple peels methanol extract APME which had lowest value of liver and kidney function parameters followed by red beet peels methanol extract RBME.
Conclusion: It can be concluded that extract of some fruits and vegetables peels (apple APME, banana BPME, red beet RBPME and potato PPME) especially methanol extract has a significant protective effect against acute hepatotoxicity induced by CCl4 in rats, which may be due to its free radical scavenging effect and its ability to increase antioxidant activity.